NAD Technical Data Sheet
Product Name: NAD
CAS No.: 53-84-9
Synonym: β-NAD, β-Nicotinamide Adenine Dinucleotide, NAD+, Coenzyme I, DPN
Document Version: V1.0
Application Scope: For Laboratory Research Only
Release Date: 2026
1. Basic Chemical Information
Test Item | Specification |
|---|
Full Name | β-Nicotinamide Adenine Dinucleotide (NAD / NAD+) |
Chemical Name | 1-((2R,3R,4S,5R)-5-((((((((2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methoxy)(hydroxy)phosphoryl)oxy)oxidophosphoryl)oxy)methyl)-3,4-dihydroxytetrahydrofuran-2-yl)-3-carbamoylpyridin-1-ium |
Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
Molecular Weight | 663.43 Da (Anhydrous) |
Classification | Endogenous coenzyme, nucleotide compound |
Biological Function | NAD+ is a key cellular coenzyme involved in cellular energy metabolism, redox reaction, DNA repair and Sirtuins regulation. Widely used in anti-aging research, metabolic mechanism, cell biology, antioxidant and pharmacological laboratory studies. |
2. Physical Characteristics
Appearance: White to off-white lyophilized crystalline powder
Odor: Odorless
Solubility: Freely soluble in sterile purified water; soluble in aqueous buffer; slightly soluble in methanol; insoluble in non-polar organic solvents
Aqueous Solution pH (1 mg/mL): 5.0 – 7.0
Hygroscopicity: Highly hygroscopic. Must be kept sealed, dry and protected from light to avoid degradation and moisture absorption
Melting Point: 140–142 ℃ (decomposition)
3. Quality Specifications (Research Grade)
Test Item | Test Method | Standard Specification |
|---|
Identity | RP-HPLC, ESI-MS | Match with standard reference; MW tolerance ±2 Da |
Purity (HPLC Area) | RP-HPLC | ≥98.0% (Typical ≥99.0%) |
Maximum Single Impurity | RP-HPLC | ≤0.50% |
Total Impurities | RP-HPLC | ≤2.0% |
Assay Content | HPLC / UV Quantitation | 98.0% – 102.0% |
Water Content | Karl Fischer | ≤1.0% |
Endotoxin | LAL Test (USP <85>) | ≤100 EU/mg |
Heavy Metals | ICP-MS | Pb,As,Cd,Hg ≤10 ppm each |
Residual Organic Solvents | GC | Compliant with ICH Q3C standard |
4. Storage & Stability Conditions
4.1 Lyophilized Powder
Long-term Storage: ≤ -80℃, sealed, dry and dark, shelf life: 24 months
Medium-term Storage: -20℃, sealed & desiccated, shelf life: 12 months
Critical Note: NAD+ is easily degraded by moisture and high temperature; avoid repeated freeze-thaw cycles
4.2 Reconstituted Aqueous Solution
5. Stock Solution Preparation
Molecular Weight: 663.43 Da
1 mM Standard Solution: Weigh 0.663 mg NAD powder, dissolve in 1 mL sterile purified water or pH 7.0–7.4 PBS buffer
Recommended Solvent: Sterile purified water / neutral PBS buffer
Operation Tip: Prepare fresh solution before experiments to prevent NAD+ hydrolysis and activity reduction.
6. Safety Handling & Disclaimer
This synthetic nucleotide raw material is slightly irritant to eyes, skin and respiratory tract.
Wear standard PPE: lab coat, nitrile gloves, safety goggles during operation.
Avoid inhalation of powder dust and direct mucosa contact.
Chemical waste shall be disposed per local environmental regulations.
Strict Disclaimer: This product is only for scientific laboratory research. Not for human consumption, clinical treatment or cosmetic formulation.
7. HPLC Analytical Test Conditions
Column: C18 Analytical Column (250×4.6mm, 5μm)
Mobile Phase A: 0.1% TFA in Water
Mobile Phase B: 0.1% TFA in Acetonitrile
Detection Wavelength: 220 nm / 260 nm
Flow Rate: 1.0 mL/min
Column Temperature: 30℃
8. Analytical Spectrum Characterization
8.1 RP-HPLC Chromatogram
Sharp, symmetric main peak with qualified impurity distribution. No obvious degradation peaks. Batch-specific HPLC chromatogram available upon request.
8.2 NMR Spectroscopy (¹H-NMR / ¹³C-NMR)
Test solvent: D₂O / DMSO-d₆, 400–500 MHz. Characteristic chemical shifts of adenine, nicotinamide and ribose groups fully match standard NAD molecular structure.
8.3 ESI-MS Identity Confirmation
Theoretical molecular weight: 663.43 Da; Expected [M+H]⁺ = 664.43 Da, mass tolerance ±2 Da, consistent with NAD standard identity.
End of Document
