TB-500

TB-500 Raw Material Technical Data Sheet

Product Name: TB-500

CAS No.: 885340-08-9

Document Version: V1.0

Application: For Laboratory Research Only

Date: 2026

Item	Specification
Full Name	TB-500 (Thymosin Beta-4 Fragment)
Synonym	N-Acetyl-Leu-Lys-Lys-Thr-Glu-Thr-Gln; Ac-LKKTETQ
Molecular Formula	C38H68N10O14
Molecular Weight	889.01 Da
1-Letter Sequence	Ac-LKKTETQ
3-Letter Sequence	Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH
Peptide Length	7 amino acids, N-terminal acetylated linear synthetic peptide
Biological Function	Regulates actin binding, promotes cell migration, angiogenesis and tissue repair, reduces inflammation

Appearance: White to off-white lyophilized powder
Odor: Odorless
Solubility: Freely soluble in sterile purified water / neutral PBS (pH 6.5–8.0); sparingly soluble in DMSO;

insoluble in non-polar organic solvents

Test Item	Test Method	Specification
Identity	RP-HPLC, ESI-MS	Consistent with reference standard; Measured MW ±2 Da
Chromatographic Purity (RP-HPLC, Area %)	RP-HPLC	≥99.0%
Largest Single Unknown Impurity	RP-HPLC	≤0.50%
Total Related Impurities	RP-HPLC	≤2.0%
Peptide Assay Content	HPLC Quantitation / Potentiometric Titration	98.0% ~ 102.0%
Water Content	Karl Fischer Titration	≤1.0%
Residual TFA / Acetate	Ion Chromatography	≤0.5%
Endotoxin (LAL, USP <85>)	Turbidimetric LAL Method	≤100 EU/mg
Heavy Metals (Pb, As, Cd, Hg)	ICP-MS	Each ≤10 ppm
Residual Organic Solvents	GC	Comply with ICH Q3C requirements
Amino Acid Composition Analysis	Amino Acid Analyzer	Within ±10% theoretical stoichiometry

Long-term storage: ≤ -80 ℃, sealed, dry, protected from light — Shelf life: 24 months
Medium-term storage: -20 ℃, sealed & desiccated — Shelf life: 12 months
Important: Avoid repeated freeze-thaw cycles

-80 ℃: Stable up to 6 months
-20 ℃: Stable up to 1 month
Do not store aqueous solution at 4 ℃ or room temperature for extended periods

Molecular Weight = 889.01

Suggestion: Prepare fresh working solution for in vivo experiments.

This synthetic peptide may irritate skin, eyes and respiratory tract.
Required PPE: Lab coat, nitrile gloves, safety goggles.
Prevent inhalation of powder dust and direct skin contact.
Waste shall be disposed as chemical waste following local regulations.
Disclaimer: THIS MATERIAL IS FOR LABORATORY RESEARCH ONLY. Not approved for human administration, clinical use or veterinary medication.

End of Document