GHK-Cu

Product Name: GHK-Cu

CAS No.: 89030-95-5

Synonym: Copper Tripeptide-1, Prezatide Copper, Glycyl-L-Histidyl-L-Lysine Copper(II) Complex

Document Version: V1.0

Application: For Laboratory Research Only

Date: 2026

1. Basic Chemical Information

Item	Specification
Full Name	GHK-Cu (Copper Tripeptide-1)
Chemical Name	[N2-(N-Glycyl-L-histidyl)-L-lysinato(2-)]copper(II)
Molecular Formula	C14H22CuN6O4
Molecular Weight	401.91 Da (1:1 copper complex)
1-Letter Sequence	Gly-His-Lys (GHK) chelated with Cu²⁺
3-Letter Sequence	H-Gly-His-Lys-OH · Cu(II)
Peptide Structure	Linear tripeptide, 1:1 chelation complex with copper(II) ion
Biological Function	Natural plasma-derived copper peptide complex; promotes collagen & elastin synthesis, antioxidant activity, supports tissue repair, wound healing, skin regeneration and anti-inflammatory research

Appearance: Light blue to blue lyophilized powder
Odor: Odorless
Solubility: Freely soluble in sterile purified water / neutral PBS (pH 6.0–8.0); soluble in DMSO; insoluble in non-polar organic solvents
pH (1 mg/mL aqueous solution): 6.0 – 8.0
Hygroscopicity: Highly hygroscopic; avoid prolonged exposure to air, moisture and strong light

Test Item	Test Method	Specification
Identity	RP-HPLC, ESI-MS	Consistent with reference standard; Measured MW ±2 Da
Chromatographic Purity (RP-HPLC, Area %)	RP-HPLC	≥99.0%
Largest Single Unknown Impurity	RP-HPLC	≤0.50%
Total Related Impurities	RP-HPLC	≤2.0%
Peptide Assay Content	HPLC Quantitation	98.0% ~ 102.0%
Water Content	Karl Fischer Titration	≤1.0%
Residual TFA / Acetate	Ion Chromatography	≤0.5%
Endotoxin (LAL, USP <85>)	Turbidimetric LAL Method	≤10 EU/mg
Heavy Metals (Pb, As, Cd, Hg)	ICP-MS	Each ≤10 ppm
Residual Organic Solvents	GC	Comply with ICH Q3C requirements
Amino Acid Composition Analysis	Amino Acid Analyzer	Within ±10% theoretical stoichiometry

Long-term storage: ≤ -80 ℃, sealed, dry, protected from light — Shelf life: 24 months
Medium-term storage: -20 ℃, sealed & desiccated — Shelf life: 12 months
Important: Avoid repeated freeze-thaw cycles, protect from light to prevent copper complex dissociation

-80 ℃: Stable up to 6 months
-20 ℃: Stable up to 1 month
Do not store aqueous solution at 4 ℃ or room temperature for extended periods; avoid light exposure

Molecular Weight = 401.91 Da

1 mM solution: 0.402 mg GHK-Cu per 1 mL solvent
Recommended solvent: Sterile purified water or pH7.0–7.4 PBS buffer
Suggestion: Gentle swirling for dissolution, do not shake vigorously; prepare fresh working solution for in vitro & in vivo experiments.

This copper-peptide complex may irritate skin, eyes and respiratory tract.
Required PPE: Lab coat, nitrile gloves, safety goggles.
Prevent inhalation of powder dust and direct skin contact.
Waste shall be disposed as chemical waste following local regulations.
Disclaimer: THIS MATERIAL IS FOR LABORATORY RESEARCH ONLY. Not approved for human administration, clinical use or veterinary medication.

**End of Document**

📄 JTX-GHK260604-COA.pdf