Dihexa
Product Name: Dihexa
CAS No.: 1401708-83-5
Synonym: PNB-0408, N-hexanoic-Tyr-Ile-(6)-aminohexanoic amide
Document Version: V1.0
Application Scope: For Laboratory Research Only
Release Date: 2026
1. Basic Chemical Information
Test Item | Specification |
|---|
Full Name | Dihexa |
Chemical Name | (2S,3S)-N-(6-amino-6-oxohexyl)-2-[[(2S)-2-(hexanoylamino)-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylpentanamide |
Molecular Formula | C₂₇H₄₄N₄O₅ |
Molecular Weight | 504.66 Da |
Structural Sequence | Hexanoyl-Tyr-Ile-Ahx-NH₂ (Modified dipeptide derivative) |
Peptide Feature | Synthetic angiotensin IV derivative, small-molecule bioactive peptide analog |
Target Receptor | c-Met / HGFR receptor |
Biological Function | Potent neuroprotective and synaptogenic compound; activates HGF/c-Met signaling pathway, promotes neuronal repair, enhances synaptic plasticity, improves cognitive function, widely used in neuroscience and brain injury mechanism research |
2. Physical Characteristics
Appearance: White to off-white lyophilized crystalline powder
Odor: Odorless
Solubility: Soluble in DMSO and methanol; sparingly soluble in sterile purified water; insoluble in non-polar organic solvents
Aqueous Solution pH (1 mg/mL): 6.0 – 8.0
Hygroscopicity: Moderately hygroscopic. Keep tightly sealed, dry and dark, avoid long-term air exposure and high humidity
3. Quality Specifications (Research Grade)
Test Item | Test Method | Standard Specification |
|---|
Identity | RP-HPLC, ESI-MS | Match with standard reference; MW tolerance ±2 Da |
Purity (HPLC Area) | RP-HPLC | ≥99.0% |
Maximum Single Impurity | RP-HPLC | ≤0.50% |
Total Impurities | RP-HPLC | ≤2.0% |
Peptide Assay Content | HPLC / Titration | 98.0% – 102.0% |
Water Content | Karl Fischer | ≤1.0% |
Residual TFA/Acetate | Ion Chromatography | ≤0.5% |
Endotoxin | LAL Test (USP <85>) | ≤10 EU/mg |
Heavy Metals | ICP-MS | Pb,As,Cd,Hg ≤10 ppm each |
Residual Organic Solvents | GC | Compliant with ICH Q3C standard |
Amino Acid Composition | Amino Acid Analyzer | Within ±10% theoretical value |
4. Storage & Stability Conditions
4.1 Lyophilized Powder (Original Package)
Long-term Storage: ≤ -80℃, sealed, dry and dark environment, shelf life: 24 months
Medium-term Storage: -20℃, sealed and desiccated, shelf life: 12 months
Note: Avoid repeated freezing and thawing cycles
4.2 Reconstituted Solution
5. Stock Solution Preparation
Molecular Weight: 504.66 Da
1 mM Standard Solution: Weigh 0.505 mg Dihexa powder, dissolve in 1 mL DMSO or sterile solvent
Recommended Solvent: DMSO (preferred), methanol, sterile purified water
Operation Tip: Fresh preparation is recommended for in vitro and in vivo neurological research assays.
6. Safety Handling & Disclaimer
This synthetic bioactive peptide analog is slightly irritant to eyes, skin and respiratory mucosa.
Wear standard PPE: lab coat, nitrile gloves, safety goggles during operation.
Avoid inhalation of powder dust and direct skin contact.
Waste materials shall be disposed in accordance with local chemical waste management regulations.
Strict Disclaimer: This product is only for scientific laboratory research. Not for human clinical use, medication or veterinary application.
7. HPLC Analytical Test Conditions
Column: C18 Analytical Column (250×4.6mm, 5μm)
Mobile Phase A: 0.1% TFA in Water
Mobile Phase B: 0.1% TFA in Acetonitrile
Detection Wavelength: 220 nm / 280 nm
Flow Rate: 1.0 mL/min
Column Temperature: 30℃
8. Analytical Spectrum Characterization
8.1 RP-HPLC Chromatogram
Sharp and symmetric main peak with no obvious impurity peaks exceeding standard limit. Batch-specific HPLC chromatogram is available for customer verification upon request.
8.2 NMR Spectroscopy (¹H-NMR / ¹³C-NMR)
Test solvent: DMSO-d₆ / D₂O, 400–500 MHz. Characteristic chemical shifts of tyrosine, isoleucine and hexanoyl modified groups are consistent with Dihexa target molecular structure. Official NMR spectrum report can be provided for bulk orders.
8.3 ESI-MS Identity Confirmation
Theoretical molecular weight: 504.66 Da; Expected quasi-molecular ion [M+H]⁺ = 505.66 Da, mass detection tolerance ±2 Da, fully matches structural identity.
End of Document
