Kisspeptin-10 Raw Material
Product Name: Kisspeptin-10 Raw Material (Human)
CAS No.: 374675-21-5
Document Version: V1.0
Application Scope: For Laboratory Research Only
Release Date: 2026
1. Basic Chemical Information
Test Item | Specification |
|---|
Full Name | Kisspeptin-10 (Human Metastin 45-54, Kp-10) |
Synonym | KiSS-1 metastasis-suppressor peptide, KISS1R agonist peptide |
Molecular Formula | C₆₃H₈₃N₁₇O₁₄ |
Molecular Weight | 1302.44 Da (free base) |
1-Letter Sequence | YNWNSFGLRF-NH₂ |
3-Letter Sequence | H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ |
Structural Feature | 10 amino acids linear peptide, C-terminal amidated modification |
Target Receptor | KISS1R (GPR54) |
Biological Function | Potent KISS1R agonist, regulates reproductive hormone secretion, hypothalamic signal transduction, cell metastasis inhibition and neuroendocrine research |
2. Physical Characteristics
Appearance: White to off-white lyophilized crystalline powder
Odor: Odorless
Solubility: Soluble in sterile purified water (≥4 mg/mL), neutral PBS buffer (pH 6.5–8.0) and DMSO; insoluble in non-polar organic solvents
Aqueous Solution pH (1 mg/mL): 6.0 – 8.0
Hygroscopicity: Moderately hygroscopic. Keep sealed and dry, avoid long-term air exposure and high humidity environment
3. Quality Specifications
Test Item | Test Method | Standard Specification |
|---|
Identity | RP-HPLC, ESI-MS | Match with standard reference; MW tolerance ±2 Da |
Purity (HPLC Area) | RP-HPLC | ≥98.0% (Typical ≥99.0%) |
Maximum Single Impurity | RP-HPLC | ≤0.50% |
Total Impurities | RP-HPLC | ≤2.0% |
Peptide Assay Content | HPLC / Titration | 98.0% – 102.0% |
Water Content | Karl Fischer | ≤1.0% |
Residual TFA/Acetate | Ion Chromatography | ≤0.5% |
Endotoxin | LAL Test (USP <85>) | ≤100 EU/mg |
Heavy Metals | ICP-MS | Pb,As,Cd,Hg ≤10 ppm each |
Residual Organic Solvents | GC | Compliant with ICH Q3C standard |
Amino Acid Composition | Amino Acid Analyzer | Within ±10% theoretical value |
4. Storage & Stability Conditions
4.1 Lyophilized Powder (Original Package)
Long-term Storage: ≤ -80℃, sealed, dry and dark environment, shelf life: 24 months
Medium-term Storage: -20℃, sealed and desiccated, shelf life: 12 months
Note: Avoid repeated freezing and thawing cycles
4.2 Reconstituted Aqueous Solution
5. Stock Solution Preparation
Molecular Weight: 1302.44 Da
1 mM Standard Solution: Weigh 1.302 mg Kisspeptin-10 powder, dissolve in 1 mL sterile solvent
Recommended Solvent: Sterile purified water / pH 7.0-7.4 PBS buffer
Operation Tip: Fresh preparation is recommended for in vivo experiments to ensure biological activity.
6. Safety Handling & Disclaimer
This product is a synthetic bioactive peptide, slightly irritant to eyes, skin and respiratory mucosa.
Wear standard PPE: lab coat, nitrile gloves, safety goggles during operation.
Avoid inhalation of powder dust and direct skin contact.
Waste materials shall be disposed in accordance with local chemical waste management regulations.
Strict Disclaimer: This product is only for scientific laboratory research. Not for human clinical use, medication or veterinary application.
7. HPLC Analytical Test Conditions
Column: C18 Analytical Column (250×4.6mm, 5μm)
Mobile Phase A: 0.1% TFA in Water
Mobile Phase B: 0.1% TFA in Acetonitrile
Detection Wavelength: 220 nm / 280 nm
Flow Rate: 1.0 mL/min
Column Temperature: 30℃
End of Document
