Thymosin α1
Product Name: Thymosin α1 Raw Material
CAS No.: 62304-98-7
Synonym: Thymosin Alpha 1, Thymalfasin, Zadaxin
Document Version: V1.0
Application Scope: For Laboratory Research Only
Release Date: 2026
1. Basic Chemical Information
Test Item | Specification |
|---|
Full Name | Thymosin α1 (Thymosin Alpha 1) |
Chemical Name | N-acetyl endogenous thymic immunomodulatory peptide |
Molecular Formula | C₁₂₉H₂₁₅N₃₃O₅₅ |
Molecular Weight | 3108.28 Da |
1-Letter Sequence | Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN |
Full Sequence | Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH |
Peptide Structure | Linear 28-amino-acid N-acetylated endogenous thymic peptide, high stability |
Biological Function | Powerful immune-modulating peptide derived from thymus tissue. Enhances T-cell and NK-cell activity, regulates immune response, improves anti-viral and anti-inflammatory capability, widely applied in immunology, anti-infection and immune mechanism research |
2. Physical Characteristics
Appearance: White to off-white lyophilized crystalline powder
Odor: Odorless
Solubility: Freely soluble in sterile purified water and neutral PBS buffer (pH 6.5–8.0); soluble in DMSO; insoluble in non-polar organic solvents
Aqueous Solution pH (1 mg/mL): 6.0 – 8.0
Hygroscopicity: Moderately hygroscopic. Keep tightly sealed, dry and dark, avoid long-term air exposure and moisture absorption
3. Quality Specifications
Test Item | Test Method | Standard Specification |
|---|
Identity | RP-HPLC, ESI-MS | Match with standard reference; MW tolerance ±2 Da |
Purity (HPLC Area) | RP-HPLC | ≥99.0% |
Maximum Single Impurity | RP-HPLC | ≤0.50% |
Total Impurities | RP-HPLC | ≤2.0% |
Peptide Assay Content | HPLC / Titration | 98.0% – 102.0% |
Water Content | Karl Fischer | ≤1.0% |
Residual TFA/Acetate | Ion Chromatography | ≤0.5% |
Endotoxin | LAL Test (USP <85>) | ≤10 EU/mg |
Heavy Metals | ICP-MS | Pb,As,Cd,Hg ≤10 ppm each |
Residual Organic Solvents | GC | Compliant with ICH Q3C standard |
Amino Acid Composition | Amino Acid Analyzer | Within ±10% theoretical value |
4. Storage & Stability Conditions
4.1 Lyophilized Powder (Original Package)
Long-term Storage: ≤ -80℃, sealed, dry and dark environment, shelf life: 24 months
Medium-term Storage: -20℃, sealed and desiccated, shelf life: 12 months
Note: Avoid repeated freezing and thawing cycles and moisture absorption
4.2 Reconstituted Aqueous Solution
5. Stock Solution Preparation
Molecular Weight: 3108.28 Da
1 mM Standard Solution: Weigh 3.108 mg Thymosin α1 powder, dissolve in 1 mL sterile purified water or PBS buffer
Recommended Solvent: Sterile purified water / pH 7.0-7.4 PBS buffer
Operation Tip: Prepare fresh working solution to maintain immune-modulating bioactivity for cell and animal experiments.
6. Safety Handling & Disclaimer
This synthetic immunomodulatory peptide is slightly irritant to eyes, skin and respiratory mucosa.
Wear standard PPE: lab coat, nitrile gloves, safety goggles during operation.
Avoid inhalation of powder dust and direct skin contact.
Waste materials shall be disposed in accordance with local chemical waste management regulations.
Strict Disclaimer: This product is only for scientific laboratory research. Not for human clinical use, medication or veterinary application.
7. HPLC Analytical Test Conditions
Column: C18 Analytical Column (250×4.6mm, 5μm)
Mobile Phase A: 0.1% TFA in Water
Mobile Phase B: 0.1% TFA in Acetonitrile
Detection Wavelength: 220 nm / 280 nm
Flow Rate: 1.0 mL/min
Column Temperature: 30℃
8. Analytical Spectrum Characterization
8.1 RP-HPLC Chromatogram
Sharp and symmetric main peak with qualified impurity level. No abnormal miscellaneous peaks, meets research-grade purity requirements. Batch HPLC report available upon request.
8.2 NMR Spectroscopy (¹H-NMR / ¹³C-NMR)
Test solvent: D₂O / DMSO-d₆, 400–500 MHz. Characteristic chemical shifts match the full 28AA acetylated peptide structure of Thymosin α1, confirming structural consistency.
8.3 ESI-MS Identity Confirmation
Theoretical molecular weight: 3108.28 Da; Expected [M+H]⁺ ion matches theoretical value with tolerance ±2 Da, verifying product identity.
End of Document
