Melanotan II
Product Name: Melanotan II
CAS No.: 121062-08-6
Synonym: MT2, MT-II, Melanotan 2, Ac-[Nle4,Asp5,D-Phe7,Lys10]-α-MSH (4-10)
Document Version: V1.0
Application Scope: For Laboratory Research Only
Release Date: 2026
1. Basic Chemical Information
Test Item | Specification |
|---|
Full Name | Melanotan II (MT2) |
Chemical Name | N-Acetyl-L-norleucyl-L-α-aspartyl-L-histidyl-D-phenylalanyl-L-arginyl-L-tryptophyl-L-lysinamide (2→7)-lactam cyclic peptide |
Molecular Formula | C₅₀H₆₉N₁₅O₉ |
Molecular Weight | 1024.18 Da |
Sequence Code | Ac-Nle-Asp-His-D-Phe-Arg-Trp-Lys-NH₂ (Cyclic Lactam Structure) |
Peptide Feature | Synthetic cyclic melanocortin analog, lactam-bridged structure, contains D-amino acid, high structural stability |
Target Receptor | MC1, MC3, MC4, MC5 Melanocortin Receptors |
Biological Function | Potent melanocortin receptor agonist, regulates melanin synthesis, modulates energy metabolism and neuroendocrine activity, widely used in pigmentation mechanism, metabolic regulation and neuroscience laboratory research |
2. Physical Characteristics
Appearance: White to off-white lyophilized crystalline powder
Odor: Odorless
Solubility: Freely soluble in sterile purified water and neutral PBS buffer (pH 6.5–8.0); soluble in DMSO; insoluble in non-polar organic solvents
Aqueous Solution pH (1 mg/mL): 6.0 – 8.0
Hygroscopicity: Moderately hygroscopic. Keep tightly sealed, dry and dark, avoid long-term air exposure and high humidity
3. Quality Specifications
Test Item | Test Method | Standard Specification |
|---|
Identity | RP-HPLC, ESI-MS | Match with standard reference; MW tolerance ±2 Da |
Purity (HPLC Area) | RP-HPLC | ≥99.0% |
Maximum Single Impurity | RP-HPLC | ≤0.50% |
Total Impurities | RP-HPLC | ≤2.0% |
Peptide Assay Content | HPLC / Titration | 98.0% – 102.0% |
Water Content | Karl Fischer | ≤1.0% |
Residual TFA/Acetate | Ion Chromatography | ≤0.5% |
Endotoxin | LAL Test (USP <85>) | ≤10 EU/mg |
Heavy Metals | ICP-MS | Pb,As,Cd,Hg ≤10 ppm each |
Residual Organic Solvents | GC | Compliant with ICH Q3C standard |
Amino Acid Composition | Amino Acid Analyzer | Within ±10% theoretical value |
4. Storage & Stability Conditions
4.1 Lyophilized Powder (Original Package)
Long-term Storage: ≤ -80℃, sealed, dry and dark environment, shelf life: 24 months
Medium-term Storage: -20℃, sealed and desiccated, shelf life: 12 months
Note: Avoid repeated freezing and thawing cycles
4.2 Reconstituted Aqueous Solution
5. Stock Solution Preparation
Molecular Weight: 1024.18 Da
1 mM Standard Solution: Weigh 1.024 mg Melanotan II powder, dissolve in 1 mL sterile purified water or PBS buffer
Recommended Solvent: Sterile purified water / pH 7.0-7.4 PBS buffer
Operation Tip: Fresh preparation is recommended to maintain cyclic peptide structural stability and biological activity.
6. Safety Handling & Disclaimer
This synthetic cyclic peptide is slightly irritant to eyes, skin and respiratory mucosa.
Wear standard PPE: lab coat, nitrile gloves, safety goggles during operation.
Avoid inhalation of powder dust and direct skin contact.
Waste materials shall be disposed in accordance with local chemical waste management regulations.
Strict Disclaimer: This product is only for scientific laboratory research. Not for human clinical use, medication or veterinary application.
7. HPLC Analytical Test Conditions
Column: C18 Analytical Column (250×4.6mm, 5μm)
Mobile Phase A: 0.1% TFA in Water
Mobile Phase B: 0.1% TFA in Acetonitrile
Detection Wavelength: 220 nm / 280 nm
Flow Rate: 1.0 mL/min
Column Temperature: 30℃
8. Analytical Spectrum Characterization
8.1 RP-HPLC Chromatogram
Sharp and symmetric main peak with no excessive impurity peaks. Batch-specific HPLC chromatogram is available for customer verification upon bulk inquiry.
8.2 NMR Spectroscopy (¹H-NMR / ¹³C-NMR)
Test solvent: D₂O / DMSO-d₆, 400–500 MHz. Characteristic chemical shifts match the cyclic lactam structure and amino acid residues of Melanotan II, fully consistent with target molecular structure.
Theoretical molecular weight: 1024.18 Da; Expected quasi-molecular ion [M+H]⁺ = 1025.18 Da, mass detection tolerance ±2 Da, meets identity confirmation standard.
End of Document
