Retatrutide

Retatrutide Raw Material

Code Name:RT CAS No.: 2381089-83-2 Version: V1.0 | For Research Use Only

Item	Specification
Full Name	Retatrutide
Synonym	RT
Molecular Formula	C221H342N46O68
Molecular Weight	4731.33 Da (free base)
Amino Acid Sequence	H-Tyr-Aib-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Ile-α-Me-Leu-Leu-Asp-Lys[C20-diacid-γ-Glu-(AEEA)-Lys]-Ala-Gln-Aib-Ala-Phe-Ile-Glu-Tyr-Leu-Leu-Glu-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH₂
Sequence Short Form	Y(Aib)QGTFTSDYSI(α-Me-Leu)LDK{Lys(AEEA-γGlu-C20 diacid)}AQ(Aib)AFIEYLLEGGPSSGAPPPS-NH₂
Target Receptors	GLP‑1R / GIPR / GCGR Triple Agonist
EC₅₀ (Human)	GLP‑1R: 0.775 nM；GIPR: 0.0643 nM；GCGR: 5.79 nM

Appearance: White to off-white lyophilized powder
Odor: Odorless
Solubility:
- Water: ≥30 mg/mL (adjust pH neutral/weak alkaline, ultrasonic assist)
- DMSO: Soluble
- Not soluble in non-polar organic solvents
pH (aqueous solution, 1 mg/mL): ~7.0–8.5
Hygroscopicity: Strongly hygroscopic; avoid long-term exposure to air

Test Item	Test Method	Specification
Identity	RP-HPLC, MS (ESI-MS)	Consistent with reference standard; Measured MW matches theoretical value ±2 Da
Chromatographic Purity	RP-HPLC	≥99.0%
Largest Single Unknown Impurity	RP-HPLC	≤0.50%
Total Related Impurities	RP-HPLC	≤2.0%
Peptide Content (Assay)	Potentiometric Titration / HPLC Quantitation	98.0% ~ 102.0%
Water Content	Karl Fischer Titration	≤1.0%
Residual Acetic Acid / TFA	Ion Chromatography	≤0.5%
Endotoxin (LAL, USP <85>)	Turbidimetric LAL	≤100 EU/mg
Heavy Metals (Pb, As, Cd, Hg)	ICP-MS	Each ≤10 ppm
Residual Organic Solvents	GC	Meet ICH Q3C limits
Amino Acid Composition Analysis	Amino Acid Analyzer	Within ±10% of theoretical stoichiometry

nitrogen blanket — Shelf life: 24 months

-80 ℃: Stable for up to 6 months
-20 ℃: Stable for up to 1 month
Do not store aqueous solution at 4 ℃ or room temperature for extended periods

Target concentration calculation basis: MW = 4731.33

Hazard: Irritant to eyes, skin and respiratory tract
PPE requirement: Nitrile gloves, goggles, lab coat
Avoid inhalation of powder dust
Waste disposal: Treat as peptide biological chemical waste following local regulations
Important Disclaimer: This raw material is FOR LABORATORY RESEARCH ONLY.

Not approved for human administration, clinical use or veterinary medication.